Microbiology

biological sciences

elementary

BI1_W01, BI1_W04,BI1_W05,BI1_W07

(in Polish) Wymagania wstępne: podstawowa wiedza z zakresu biologii, chemii ogólnej i biochemii

Level of course: basic

Course objectives: The purpose is to familiarize students with the general principles for growth,evaluation and classification of microorganisms, description of microbial life forms, uses of microorganisms and their role in biotechnology, environment prptection, and impact on human health. During laboratory practice students will learn basic microbiological laboratory techniques.

Prerequisites: bacis knowledge of general biology, general chemistry and biochemistry

Full course description

Course contents:

Lectures:

1. Procaryotes and Eucaryotes. Bacterial cell morphology.

2. Viruses - occurrence and structure.

3. Fungi - taxonomy, structure and their role in environment.

4. Microorganisms - principle of growth, development, nutrition.

5. Fermentation, aerobic and anaerobic respiration.

6. Chemolithotrophic and phototrophic bacteria.

7. Industrial microbiology nad Biotechnology (the production of organic acid, antibiotics, mycotoxins, vitamines, enzymes)

8. Microorganisms and plant protection - biopreparations and their use in plant protection.

9. The role of microorganisms in the decomposition of organic matter and in the cycle of different elements.

10. Genetic diversity of microorganisms.

11. Interactions between microorganisms - both direct and indirect impacts.

12. Interaction between the environment and microorganisms (plants, animals, limiting environment factors)

13. Microorganisms and water and ground purifiction (wasterwater, contaminated ground)

14. Foods microbiology - contamination of raw materials and food products, health risks.

15. Microorganisms and human health. Pathogenic microorganisms and diseases.

Laboratory sessions:

1. The rules of practice and principles of work safety in the laboratory. Basic glass and equipment for microbiological lab. Microbiological methods of sterilization.Media and methods of microorganisms cultivation.Microbiological observations.

2. Isolation of microorganisms from different sourses (water, soil). Methods of inoculation on solid medium.

3. Microscopic observation of different forms of microorgannisms. Isolation of pure culture.

4. Morphology of bacteria. Staining methods.

5. Morphology of fungi.Cells and colonies of yeas and mold )

6. Detection of redox and proteolytic activity of bacteria. Bacterial activity and lactic acid fermentation

7. Rapid biochemical and serological methods for pathogenic bacteria assessment.

8. Effects of temperature and selected chemicals on the growth of microorganisms.

9. Interactios between microorganisms. Types of indirect impacts.

10. Determination of microbial sensivity to antibiotics using disks diffusion methods and agar well diffusion plate methods.

11. Sewage and their purification. Microbiological analysis of the water using a membrane filtration methods. Microscopic analysis of activated sludge from wastewater.

12. Methods for quantification of bacteria and fungi. The microbiological analysis of air by Koch sedimantetion method.

13. Transformation in bacteria. Part 1 - Inoculation of transformed and non-transformed bacteria on solid medium

14. Transformation in bacteria. Part 2 - Presentation of results.

15. Final exam

Assesment methods: Final exam consisting of combination of multi-choice and short written answer

Obligatory literature:

Błaszczyk M.K. - Environmental microbiology, PWN, Warszawa 2019 (in polish)

Kunicki-Goldfinger W.J.H. - Life of bacteria, PWN, Warszawa 2007 (in polish)

Libudzisz Z., Kowal K., Żakowska Z. - Technical microbiology t. 1 i 2, PWN, Warszawa 2019 (in polish)

Salyers A.A., Whitt D.D. - Microbiology, Diversity, disease and environment, PWN, Warszawa 2012

Schlegel H.G. - General microbiology, PWN, Warszawa 2008 (in polish)

Baj J., Markiewicz Z. ed. - Molecular biology of bacteriai, PWN, Warszawa 2012 (in polish)

Supplementary literature:

Alberts B., Bray D., Hopkins K., Johnson A., Lewis J., Raff M., Roberts K., Walter P. - Basic of cell biology, PWN, Warszawa 2016

Błaszczyk M.K. - Microorganisms in environment protection, PWN, Warszawa 2009 (in polish)

Klimiuk E., Łebkowska M. - Biotechnology in environment protection, PWN, Warszawa 2008 (in polish)

Ratledge C., Kristiansen B. - Basic Biotechnologyi, PWN, Warszawa 2019

Knowledge:

Lo 1 - student be able to understand basic microbiological phenomena and process

Lo 2 - student has knowledge of basic conceptual categories and microbiological terminology and is familiar with the development of microbiology and the research methods used in it

Lo 3 - student has knowledge of the basic techniques and research tools used in microbiology

Lo 4 - student knows the basic principles of health and safety in microbiological laboratory

Skills:

Lo 5 - student uses the basic techniques and research tools used in the mucrobiological laboratory

Lo 6 - student performs simple experiments with microorganisms in the laboratory under the supervision of a scientific supervisor

Competencies:

Lo 7 - student is able to interact and work in a group, taking on different roles

Subject effects for lecture (EK 1-2), subject effects for exercises (EK 3-7)

Student activity student workload in hours

participation in lecture 30

participation in laboratory 45

preparation for laboratories 5

time to write the report 15

prepare for the test 15

prepare for the exam 20

TOTAL HOURS 130/30(25) hrs. ≈4

Number ECTS: 4

Assessment criteria:

Knowledge (Learning outcomes 1-2)

Grade 2 (fail): the student does not understand the basic microbiological phenomena and processes, has very limited knowledge of the basic conceptual categories and microbiological terminology and applied research methods,

Grade 3 (sufficient): the student understands to a limited extent basic phenomena and microbiological processes, has limited knowledge about the basic conceptual categories and microbiological terminology and applied research methods,

Grade 4 (good): the student knows fully the basic phenomena and microbiological processes, has an average knowledge of basic conceptual categories and microbiological terminology and applied research methods,

Grade 5 (excellent): has full knowledge of the basic phenomena and microbial processes, knows fully the basic conceptual categories and terminology of microbiology and used in the research methods,

The condition for taking the exam is to pass the course in the laboratory. The written examination will be a combination of multiple-choice test, replenishing the missing text and answer the questions.

Exam grade: 1-50 points (26-30 points. - 3.0; 31-35 points. - 3.5; 36-40 points. - 4.0; 41-45 points. - 4.5; 46 -50 points. - 5.0)