Molecular Biology (practice)

The aims of teaching: to present basics of molecular biology and the main topics of this discipline, including macroparticles and macriparticle complexes of DNA, RNA and proteins, and presentation of experimental technologies used in investigations and manipulations with these particles.

The course includes practice, that is realized in few blocks.The first block is focused on techniques of DNA analysis: methods of isolation of plasmid DNA, polymerase chain reaction (PCR), PCR products separation on agarose gels. In the second block students perform restriction digest of PCR products and analysis of DNA fragments separated in agarose gels, as well as learn to draw restriction maps. In the next block students isolate DNA from agarose gels, perform the ligation of plasmid DNA with DNA insert as well as transformation of competent bacteria with the prepared vector. The fourth block of Practice allows students to know techniques of working with RNA: isolation, assessment of quality and quantity, RNA separation in agarose gels. The next two blocks of Practice are focused on proteins. Students perform proteins isolation, learn how to assess the quantity of isolated protein, perform protein separation in polyacrylamide gels that are next stained with two different methods. Analysis of individual protein in the isolate with Western Blot method is also performed. At the end of the block of Practice students familiarize with databases: they learn searching information on different organisms, genes, sequences as well train the technique of primer design, analysis of restriction sites and comparison of different DNA sequences. Students also familiarize with methodology of computational analysis of results of DNA sequencing with Sanger method and pyrosequencing.

"Genomes" T.A. Brown,

Józef Kur, Podstawy Inżynierii Genetycznej, Gdańsk,

Ryszard Słomski, Przykłady analiz DNA, Poznań,

Kłyszejko -Stefanowicz L. (red.) - "Ćwiczenia z biochemii" PWN S.A.,

BI1_W01 student understands basic phenomena and biological processes, and especially structure and function of cells and macromolecules, structure of proteins and nucleic acids, variability of structure as well as experimental technologies used in investigations and manipulation with these macroparticles

BI1_W04 student has knowledge on the basic problems from different areas of biology and understands their relationships to the topic of molecular biology

BI1_W05 student knows basic terms from the topic of molecular biology as well as is familiar with the development of molecular biology as scientific discipline, including different techniques used in molecular investigations

BI1_W07 student knows basic investigative technics used in molecular biology

BI1_W08 student understands relationships between achievements in biology, and especially in molecular biology and possibilities of their applications in social and economic development including balanced usage of biological diversity

BI1_W09 student knows basic principles of industrial safety in the molecular biology laboratory

The final appraisal is based on the mean result of two intrasemestral tests including open and test questions. The criteria of assessment: <55%: 2 (failed); 55%-75%: 3 (third), 75%-90%: 4 (second); >90%: 5 (first). The final appraisal includes also assessment of student preparation to practice as well as activity during classes.

not applicable